In the southern outskirts of Santiponce, this fortified Gothic monastery was founded in 1301 by Guzmán El Bueno (hero of the 1294 battle at Tarifa). Over the centuries it hosted a succession of religious orders, including, in the 15th century, the hermetic Hieronymites who embellished the Patio de Evangelistas with some striking murals and Mudéjar-style floral and geometric patterns. Also outstanding is the church's altarpiece by 17th-century Sevillan sculptor Juan Martínez Montañés.
The monastery also enjoys celebrity as the place where the Bible was first translated into Castilian in 1569.